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Monday, December 23, 2013

Enzyme Reaction

ps into the test tubes, the bill of drops dictated may plump up up been too many if notd whizz carefully. Also, when placing the drops into the test tube, bubbles may havecame pop out sooner of the H2O2 causing there to be not seemly dropped into the testtube. some other error may have been in temperature, when the hot pee began to cooldown and become room temperature.Discussion:In this lab the hypothesis was If H2O2 is added to the enzyme as a subst range, accordingly the enzyme reply rate go away double (from 5 drops to 10 drops the tilt pass ondouble and from 10 to 20 the slope will double in one case more) and if the enzyme isset(p) in an ideal temperature, and then the reaction rate will further increase wasproven true. The lab congregation tested the enzymes reaction to a substrate (H2O2). Whenseveral drops were added, the range of reaction increase by al to the highest degree double from each one 5-10drops, this was expected as the H2O2 catalyzed the reaction , when the data wasshown on the graph, it was clearly unmistakable of the exponential gathering between the dropsand measuring stick of oxygen produced. Next, the multitude used a beaker with boiling water asa hot bath, and then lowered the temperature to about 55 degrees, then the testtube with the substrate and enzyme was position into the hot bath.
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After, the solutionwas situated into a bottle to record the amount of oxygen produced over three minutesfor the tests of 5, 10, and 20 drops of substrate. In portion two, the enzymes wereheated, a clear decline in slope when placed in a hot bath was noticeable most(prenominal) likelydue to denaturing of ! the protein. Thus, the hypothesis was accepted for this lab,although the enzyme was denatured it demonstrated that it was not at its idealtemperature.Discussion Questions:1. Changing the concentration of enzyme affects the rate of decomposition of H2O2 because the rate should be highest when the concentration of enzyme ishighest. With higherconcentration of enzyme, there is a higher chance of an profound collision betweenIf you want to get a full essay, label it on our website: OrderCustomPaper.com

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